![]() Ion 316 chips were utilised with the Ion Torrent semi-conductor platform Personal Genome Machine (PGM) to perform tandem sequencing of mitochondrial genomes from the core pedigree (n = 315) of the Norfolk Island Health Study. We summarise an efficient and cost-effective NGS approach for mitochondrial genomic DNA in humans using the Ion Torrent platform, and further discuss our bioinformatics pipeline for streamlined variant calling. The implementation and popularity of next generation sequencing (NGS) has led to the development of various rapid whole mitochondrial genome sequencing techniques. Griffiths, Conceptualization, Project administration, Resources, Supervision, Writing – review & editing 1, * Haupt, Conceptualization, Investigation, Methodology, Project administration, Supervision, Writing – review & editing, 1 and L. Lea, Formal analysis, Supervision, Visualization, Writing – review & editing, 1 L. Allcock, Methodology, Writing – review & editing, 3 R. Sutherland, Conceptualization, Methodology, Supervision, Writing – review & editing, 1 R. Connell, Methodology, Writing – review & editing, 1 H. Eccles, Formal analysis, Writing – review & editing, 1 J. Benton, Formal analysis, Methodology, Visualization, Writing – review & editing, 1, ¤b D. Stuart, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, 1, ¤a M. Albury, Investigation, Methodology, Writing – original draft, # 1 S. Harvey, Conceptualization, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing, # 1, 2 C.
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